The Effects of Exercise Training on Glucose Intolerance in Shrsp

s are presented in the alphabetical order of the first author names and are printed without editing in the submitted form. The Editorial Office of Physiological Research disclaim any responsibility for errors that may have been made in abstracts submitted by the authors. The 47 Scientific Meeting of the Japanese Society for Hypertension – Related Disease Model Research September 6-7, 2011, Sapporo, Japan Vol. 61 Physiol. Res. 2012 1P THE EFFECTS OF EXERCISE TRAINING ON GLUCOSE INTOLERANCE IN SHRSP Shinya Aoyama, Kumiko Takemori, Hisanori Kato, Tetsuo Murakami Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan, Department of Food Science and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan, Corporate Sponsored Research Program ‘Food for Life’, University of Tokyo, Tokyo, Japan Spontaneously hypertensive stroke-prone rats (SHRSP) exhibit not only hypertension and stroke but also insulin resistance and glucose intolerance, which have been shown to be pathologies of diabetes. One of the features of glucose intolerance in SHRSP is hypoinsulinemia. Although exercise training is reported to improve diabetes, the effects of exercise training on glucose intolerance as seen in SHRSP are not known. Therefore, the present study was conducted to evaluate the effects of exercise training on glucose tolerance, and to elucidate detailed responses to exercise through analyzing insulin signalingrelated factors in exercise-trained SHRSP. Male SHRSP at 11 and 4 weeks of age were used as adult and young rats, respectively. Preliminary experiments from our laboratory have shown that young SHRSP exhibit normal glucose tolerance, while adult SHRSP exhibit glucose intolerance. SHRSP of each age were divided into two groups, the control (Cont) group and the exercise training (Tr) group. Each Tr rat was subjected to swimming training with a river-pool device (spending 60 min/day, 5 days/week in the pool). At 14 and 9 weeks of age, each rat was given an oral-glucose tolerance test (OGTT). One week later, each rat was sacrificed under anesthesia. Isolated gastrocnemius muscles were used for the analyses of the expression of insulin signaling-related factors, including insulin receptor β subunits (IR-β), Akt, glucose transporter 4 (GLUT4) and hexokinase 2 (HXK2). Adult SHRSP: The body weight of rats in the Cont group was not different from that of rats in the Tr group. The systolic blood pressure of the Tr group was lower than that of the Cont group. Although the insulin-area under the curve (AUC) of the Cont group did not differ from that of the Tr group, glucose-AUC of the Tr group was lower than that of the Cont group. The protein content of Akt, GLUT4 and HXK2 in gastrocnemius muscle was increased by swimming training. Young SHRSP: The body weight of rats in the Tr group was lower than that of rats in the Cont group. The systolic blood pressure, glucose and insulinAUC were not different between the Cont and Tr groups. The protein content of IR-β and Akt in gastrocnemius muscle in the rats that underwent swimming training were higher than those in the Cont group. The mRNA expression of GLUT4 in gastrocnemius muscle of the Tr group tended to be higher than that in the Cont group. These results suggested that exercise training ameliorates glucose intolerance in adult SHRSP through up-regulation of insulin signaling-related factors. The results also suggested that normal glucose tolerance as seen in young SHRSP was responsible for the lack of any effect of exercise training on glucose tolerance. The results of this study should help researchers to clarify the benefit of exercise for hypertensive diabetics. THE EXAMINATION OF INHIBITORY EFFECT OF CAFFEIC ACID PHENETHYL ESTER (CAPE) TO HYPERTROPHIC